EzPCR™ FAST 2X Premix (96 tube)
More Information
96 tube (10 ㎕)
50,000 원
이 분류에 등록된 다른 상품이 없습니다. 확대보기
리뷰 위시 0

상품간략정보 및 구매기능

판매가격 판매중지
배송비결제 주문시 결제

상품의 재고가 부족하여 구매할 수 없습니다.


   EzPCR™ FAST 2X PCR Premix is a ready-to-use premix containing all the components essential for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed simply by adding primer pair and template. As Premix is supplied as a 2X concentration format, users should adjust a final reaction to 1X (if final reaction volume is 20 ㎕, 10 ㎕ of 2X Premix should be added). 

   One unit of rTaq Plus DNA Polymerase is contained in 10 ㎕ of 2X Premix. rTaq Plus DNA Polymerase is a thermostable DNA polymerase that designed for a reliable amplification of long and complex targets with a robust yield in an extremely short extension time. Due to its high elongation rate (about 100 bp/sec) and high processivity (about 250 bases), long sized products can be generated within relatively shorter running time compared to any other commercially available enzymes (5 kbp within 60 sec extension). rTaq Plus DNA Polymerase has no detectable exonuclease activity (5´→3´ or 3´→5´) and generates a 3´dA overhang, suitable for a TA cloning of PCR products. 



- Fast PCR 


- Amplification of cDNA and genomic DNA 

- Colony PCR


Qualifying Test

    Performance test, exo/endo nuclease contamination test, stability test. genomic DNA contamination test. 


Storage Condition

   Store at -20 ℃. 

   EzPCR™ FAST 2X PCR Premix is stable for at least 1years at recommended storage condition.



   - 5’ → 3’ exonuclease : no 

   - 3’ → 5’ exonuclease : no 

   - strand displacement : no 


Standard Experiment Protocol

1. Prepare the 20 ㎕ PCR solution as follows


EzPCR FAST 2X Master Mix                      10  

Primer (10 pmol/㎕)                           each 0.5 ㎕

Template DNA                                  0.1 - 10 ng
PCR grade distilled water                            - ㎕


Add distilled water to make 20 ㎕ final volume.


2. Set PCR cycling as follows

Initial denature at 95 ℃ : 3 min



 < 1 Kbp

  1 - 10 Kbp


 95 ℃

 5 sec

 10 sec


 Tm - 4 ℃

 5 sec

 10 sec


 72 ℃

 5 sec

 20 sec / Kbp

* Set 25-35 PCR cycles for effective amplification.


3. You can analyze PCR products by direct loading into agarose gel because PCR Master Mix contains   glycerol and bromophenol blue (blue color) essential for a gel loading.




1. No products

 - Confirmyourtemplateisintact:Try another reaction with a result assured primer pair and templates.


2. Smear bands or smeared background

 - Reduce template concentration: High concentration of template can lead to smearing of PCR products. Generally, 1 - 10 ng of plasmid DNA  Is working well

 - Increase annealing temperature

 - Set up a reaction mix on ice


3. Non-specific bands

 - Increase annealing temperature

 - Consider using PCR additives, like 1-2% DMSO or 0.5 - 1 X Q Buffer

 - Confirm specificity of your primers

 - Adjust concentration of template


4. Low yield

 - Increase PCR cycle number

 - Be sure appropriate concentration of your template is added. 

  • 등록된 내용이 없습니다.
Related Products

선택하신 상품이 장바구니에 담겼습니다.

계속구매 장바구니 이동

사이트 정보

회사명 : 회사명 / 대표 : 대표자명
주소 : OO도 OO시 OO구 OO동 123-45
사업자 등록번호 : 123-45-67890
전화 : 02-123-4567 팩스 : 02-123-4568
통신판매업신고번호 : 제 OO구 - 123호
개인정보관리책임자 : 정보책임자명

Copyright © 소유하신 도메인. All rights reserved.