Description

  EzPCR™ FAST 2X PCR Premix is a ready-to-use premix containing all the components essential for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed simply by adding primer pair and template.

  As Premix is supplied as a 2X concentration format, users should adjust a final reaction to 1X (if final reaction volume is 20 ㎕, 10 ㎕ of 2X Premix should be added). One unit of rTaq Plus DNA Polymerase is contained in 10 ㎕of 2X Premix.

  rTaq Plus DNA Polymerase is a thermostable DNA polymerase that designed for a reliable amplification of long and complex targets with a robust yield in an extremely short extension time. Due to its high elongation rate (about 100 bp/sec) and high processivity (about 250 bases), long sized products can be generated within relatively shorter running time compared to any other commercially available enzymes (5 kbp within 60 sec extension).

  rTaq Plus DNA Polymerase has no detectable exonuclease activity (5´-3´ or 3´-5´) and generates a 3´dA overhang, suitable for a TA cloning of PCR products.

 

Applications

 - Fast PCR

 - RT-PCR

 - Amplification of cDNA and genomic DNA

 - Colony PCR

 

Qualifying Test

Performance test, exo/endo nuclease contamination test, stability test. genomic DNA contamination test.

 

 

Features

 - 5’ → 3’ exonuclease : no

 - 3’ → 5’ exonuclease : no

 - strand displacement : no

 

Storage Condition

Store at -20 ℃ (stable up to 12 months).

Avoid repeating freeze-thaw cycle.