Ulp1 (Ub1-specific protease) is a yeast SUMO protease involved in hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-/-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, result in its maturation.The catalytic portion (403-621 aa) of Ulp1 has been used for cleavage of recombinant SUMO fusion protein to make native protein production. Since the protease recognizes the tertiary structure of SUMO protein, it cleavea the junction of the SUMO and interesting protein in a highly specific manner.Ulp1 (403-621 aa) was over-expressed and purified from E.coli strain as His-tagged form.
Features- Removal of SUMO tag from recombinant fusion protein.- Highly specific cleavage.- Broad range of active temperature (4°C ~ 37°C). Concentration & Storage Condition- 10 unit/㎕. Store at -20℃. Storage Buffer- 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM DTT, 50% glycerol. - 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 10% glycerol.
Unit Definition- One unit is defined as the amount of enzyme required to cleave > 85% of 100 ㎍ of SUMO-fusion protein at 37°C for 1 hour or 4°C overnight. QC Tests
- Activity test, SDS-PAGE purity.