SENP2 peptidase is one of SUMO-specific protease (SENP) family in human, and involved in the SUMO conjugation through the processing of SUMO1, SUMO2, and SUMO3 to mature form and deconjugation of SUMOs from targeted proteins.SENP2 has known to down-regulate CTNNB1 levels and thereby modulate the Wnt pathway Like Ulp1, SENP2 hydrolysis the peptide bond in the sequence Gly-Gly-!Ala-Thr-Tyr at the C-terminal end of SUMOs.The catalytic domain (363-588 aa) of SENP2 is capable of removal of SUMO tag from recombinant fusion protein. SENP2 was over-expressed and purified from E.coli strain as His-tagged form.
Features- Removal of SUMO tag from recombinant fusion protein.- Highly specific cleavage.- Broad range of active temperature (4°C ~ 37°C). Concentration & Storage Condition- 10 unit/㎕. Store at -20℃. Storage Buffer- 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM DTT, 50% glycerol. Reaction condition- 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 10% glycerol. Unit Definition- One unit is defined as the amount of enzyme required to cleave > 85% of 100 ㎍ of SUMO-fusion protein at 37°C for 1 hour or 4°C overnight. QC Tests
- Activity test, SDS-PAGE purity.