O-Glycosidase (Endo-α-N-acetylgalactosaminidase) hydrolyze the unsubstituted Gal-β1,3-GalNAc core disaccharide attached to serine or threonine residues of glycopeptides to afford free oligosaccharides.Recombinant Endo-α-N-acetylgalactosaminidase is expressed in E.coli containing the gene of Streptococcus pneumoniae.O-Glycosidase has a molecular weight of ~180 kDa.
Product Components- O-Glycosidase : 200 ㎕ (50,000 unit/㎕)- 10x Reaction Buffer : 500 mM sodium citrate, pH 5.5. Unit Definition- One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-2-acetamido 2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside per min at 37°C, pH 5.0. Specific Activity- 50,000 unit/㎕ , 50,000 unit/㎍ Storage Buffer- 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM EDTA, 50% glycerol. - Store at -20℃.
- Activity, SDS-PAGE purity, performance tests.