α2-3,6,8 Neuraminidase (Sialidase) catalyzes the hydrolysis of α2-3, α2-6, and α2-8 linked N-acetyl-neuraminic acid residues from glycoproteins and oligosaccharides.
Neuraminidase is purified from E. coli containing gene from Clostridium perfringens.α2-3,6,8 Neuraminidase has a molecular weight of ~43 kDa.The optimal pH is 6.0.Substrate Specificity
Unit Definition- One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide (4-MU-NANA) per min at pH 6.0 at 37 °C.Specific Activity- 100 unit/㎕, 200 unit/㎍Storage Buffer- 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM EDTA.Product Components- α2-3,6,8 Neuraminidase : 100 ㎕ (100 unit/㎕)- 10x Reaction Buffer : 500 mM sodium citrate, pH 6.0.Storage Temperature- Store at -20℃.QC Tests- Activity, SDS-PAGE purity, performance tests.
