α2-3 Neuraminidase catalyzes the hydrolysis of α2-3 linked N-acetyl-neuraminic acid residues from oligosaccharides.
α2-3 Neuraminidase is purified from E.coli containing gene from Salmonella typhimurium.It has a 260-fold kinetic preference for the α2-3 linked sialic acid linkage.α2-3 Neuraminidase has a molecular weight of ~43 kDa.The optimal pH is 6.0.Substrate Specificity- Neu5AC α2 / 3RUnit Definition- One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide (4-MU-NANA) per min at pH 5.0 at 37 °C.Specific Activity- 100 unit/㎕, 200 unit/㎍Storage Buffer- 20 mM Tris-HCl, pH7.5, 50 mM NaCl, 5 mM EDTA.Product Component- α2-3 Neuraminidase : 100 ㎕ (100 unit/㎕)- 10x Reaction Buffer : 500 mM sodium citrate, pH 6.0.
Storage TemperatureQC Tests- Activity, SDS-PAGE purity, performance tests.
