HiPi RT-PCR Kit is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) for detection of specific genes from either total RNA or polyA⁺ RNA.

HiPi RT-PCR Prime Kit provides reagents sufficient for 50 RT reactions and 500 PCR reactions.

The kit contains M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase RNase H⁻ and HiPi plus thermostable DNA polymerase. Both random hexamers and oligo d(T)15 are included, allowing cDNA synthesis from any RNA source.

The kit is optimized for 20 ㎕ RT reaction and each cDNA synthesis may be divided and used in up to 20 separate PCR reactions.

The kit contains total RNA and primer set for a positive control experiment.

Applications
- First strand cDNA synthesis from total RNA or polyA⁺ RNA, and subsequent PCR reaction.

QC tests
- Performance tests.

Storage Condition

- Store at -20℃. 

 

Kit Component

- M-MLV Reverse Transcriptase, RNase H⁻ : 10,000 unit (200 unit/㎕)
- 5x Reverse Transcription Buffer : 500 ㎕
- HiPi Plus Thermostable DNA Polymerase : 500 unit (5 unit/㎕)
- 10x HiPi Plus Reaction Buffer : 1 ml
- 10 mM dNTP : 1 ml
- Random Hexamer : 50 ㎕ (100 pmol/㎕)
- Oligo d(T)15 : 50 ㎕ (100 pmol/㎕)
- Recombinant RNase Inhibitor : 2,500 unit (50 unit/㎕)
- Total RNA, Human 293 cell : 10 ㎕ (1 ㎍/㎕)
- Control Primer Pair Mix, β-actin : 20 ㎕ (10 pmol/㎕)

- RNase-Free Water : 1 ml