HiPi RT-PCR Kit is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) for detection of specific genes from either total RNA or polyA⁺ RNA.HiPi RT-PCR Prime Kit provides reagents sufficient for 50 RT reactions and 500 PCR reactions.The kit contains M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase RNase H⁻ and HiPi plus thermostable DNA polymerase. Both random hexamers and oligo d(T)15 are included, allowing cDNA synthesis from any RNA source.The kit is optimized for 20 ㎕ RT reaction and each cDNA synthesis may be divided and used in up to 20 separate PCR reactions.The kit contains total RNA and primer set for a positive control experiment.
Applications- First strand cDNA synthesis from total RNA or polyA⁺ RNA, and subsequent PCR reaction. QC tests- Performance tests.
- Store at -20℃.
- M-MLV Reverse Transcriptase, RNase H⁻ : 10,000 unit (200 unit/㎕)- 5x Reverse Transcription Buffer : 500 ㎕- HiPi Plus Thermostable DNA Polymerase : 500 unit (5 unit/㎕)- 10x HiPi Plus Reaction Buffer : 1 ml- 10 mM dNTP : 1 ml- Random Hexamer : 50 ㎕ (100 pmol/㎕)- Oligo d(T)15 : 50 ㎕ (100 pmol/㎕)- Recombinant RNase Inhibitor : 2,500 unit (50 unit/㎕)- Total RNA, Human 293 cell : 10 ㎕ (1 ㎍/㎕)- Control Primer Pair Mix, β-actin : 20 ㎕ (10 pmol/㎕)
- RNase-Free Water : 1 ml