One-step RT-PCR 2x Premix is a mixture of thermostable
DNA Polymerase, thermostable M-MLV reverse
transcriptase, dNTPs, reaction buffer, and stabilizer. Users
can perform RT-PCR by simply adding total RNA or poly(A)
RNA as substrates and specific primer pair to the premix.
One-step RT-PCR 2x Premix also contains glycerol and
bromophenol blue as a tracking dye, enabling a direct
analysis of PCR products on an analytic gel
electrophoresis. Stabilizer in premix enables prolonging a
shelf life at -20℃. You do not need to dissolve the mixture
prior to RT-PCR reaction as does competitor’ freeze-dried
one. The composition is optimized to reduce non-specific
amplification and to minimize primer-dimer formation.
One-step RT-PCR for gel analysis.
One-step RT-PCR 2x Premix, 10㎕ in 12 x 8 strip tubes.
Performance test, exo/endo nuclease contamination test,
For long term storage, we recommend to store at -20℃
(stable up to 6 months).
Standard Experiment Protocol
1. Thaw the One-step RT-PCR 2x Premix
2. Prepare the 20 ㎕ of RT-PCR reaction mix as follow.
One-step RT-PCR 2x Master Mix 10 ㎕
Primer up-stream (10 pmol/㎕) 0.5-1 ㎕
Primer down-stream (10 pmol/㎕) 0.5-1 ㎕
Template RNA (1 – 100 ng) variable
Add distilled water to make 20 ㎕ final volume.
3. After mix and put the tubes on the PCR machine.