Pepti-Gel peptide PAGE analysis kit can be substituted for Tricine Gel system to separate a small peptide (MW 2-30 kDa) in a polyacrylamide gel electrophoresis.By using Pepti-Gel peptide PAGE analysis kit, users can obtain highly resolved results without changing Tris-Gly-SDS buffer system.The minimizing heat generation during electrophoresis using Pepti-Gel peptide PAGE analysis kit results in faster migration time and eliminating the smiling effect of bands commonly observed in Tricine gel system.The following materials are not included in the kit and should be prepared before making gel cast.0.25 M Tris-Cl (pH6.8), TEMED, 10% (w:v) ammonium persulfate, protein sample buffer, Tris-Gly-SDS running buffer.
- Store at 4℃ for 1 year
- Pepti-Gel 2x Running Gel Buffer : 100 ml (EBA-1053), 500 ml (EBA-1054)
- Shin B, Oh H, Park SM, Han HE, Ye M, et al. (2014) Intracellular Cleavage of Amyloid β by a Viral Protease NIa Prevents Amyloid β-Mediated Cytotoxicity. PLOS ONE 9(6): e98650. https://doi.org/10.1371/journal.pone.0098650