Gradi-Gel II gradient PAGE analysis kit is a novel product of ElpisBiotech to make up for defects of conventional gradient gel preparation.

Preparation of conventional gradient gel using a gradient former is a time consuming and tedious job itself. Furthermore, hand-made gradient gel is not reproducible in electrophoretic results and sometimes results in distorted images because of difference in polyacrylamide gel percentage between upper and lower part.

Gradi-Gel II gradient PAGE analysis kit uses single percentage polyacrylamide gel employing the chemical gradient formation during gel electrophoresis instead of mechanical gradient.

Gradi-Gel II gradient PAGE analysis kit is composed of 2x running gel buffer and 2x stacking gel buffer. By simply mixing buffers and acrylamide solution in kit, users can prepare a linear migrating gradient gel without unnecessary labor.

Gradi-Gel II gradient running gel is overlaid with a lower percentage stacking gel, ensuring highly separating performance and provide you with highly resolved razor-sharp protein banding.

Gradi-Gel II gradient PAGE analysis kit is completely compatible with conventional Tris-Glycine-SDS running buffer for denaturing PAGE, and Tris-Glycine for native and non-denaturing PAGE.

- Convenient, ready-to-use, reproducible gradient polyacrylamide gels
- High resolution (razor-sharp bands)
- Fast running speed (1.5-2 hrs at 100 V constant voltage)
- No need to buffer change (Tris-Glycine-SDS buffer system)
- Small peptide analysis using low percentage gels
- Optimized composition for western transfer and blotting, brilliant blue and silver staining of gels
- No distortion and shrinking of dried gels (because of single % gradient gel)Storage

- Store at 4℃ for 1 year 

 

Kit Components

- Gradi-Gel II 2x Stacking Gel Buffer : 20 ml

- Gradi-Gel II 2x Running Gel Buffer : 100 ml 

 

References

 

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• Lu Y, Yoo WG, Dai F, Lee JY, Pak JH, et al. (2018) Characterization of a novel organic solute transporter homologue from Clonorchis sinensis. PLOS Neglected Tropical Diseases 12(4): e0006459. https://doi.org/10.1371/journal.pntd.0006459

 

• Kim, S., Shin, H., Lee, G., Kim, D., Kim, H., Park, J. ... Um, J. (2015). Beneficial effects of the traditional medicine Igongsan and its constituent ergosterol on dextran sulfate sodium-induced colitis in mice. Molecular Medicine Reports, 12, 3549-3556. https://doi.org/10.3892/mmr.2015.3824

 

• Kim, H. Y., Lee, D. K., Chung, B. R., Kim, H. V., Kim, Y. Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits. J. Vis. Exp. (109), e53308, doi:10.3791/53308 (2016).

 

• Yoon et al. (2015) A vesicular stomatitis virus glycoprotein epitope-incorporated oncolytic adenovirus overcomes CAR-dependency and shows markedly enhanced cancer cell killing and suppression of tumor growth. Oncotarget. 2015; 6:34875-34891. https://doi.org/10.18632/oncotarget.5332

 

• Il Lee, Byung & Seok Suh, Yoon & Jung Chung, You & Yu, Kweon & Beum Park, Chan. (2017). Shedding Light on Alzheimer’s β-Amyloidosis: Photosensitized Methylene Blue Inhibits Self-Assembly of β-Amyloid Peptides and Disintegrates Their Aggregates. Scientific Reports. 7. 10.1038/s41598-017-07581-2.