In polyacrylamide gel electrophoresis, low % gel or gradient gel systems are commonly used to separate high molecular weight proteins (100-400 kDa). But everybody feel the difficulty in handling the low % gel and result in poor resolution.

Elpis Biotech’s Gradi-Gel I high-size PAGE analysis kit is optimized for increasing mobility of high molecular weight proteins when compared with the conventional SDS-PAGE (see migration pattern).
By using Gradi-Gel I high-size PAGE analysis kit, you can easily separate high sized proteins in easy-to -handle % of acrylamide gels.

The kit’s components are convenient to use, and also provide highly resolved results of proteins.

- Convenient, ready-to-use, reproducible results
- High resolution (razor-sharp bands)
- Fast running speed (1.5-2 hrs at 100 V constant voltage)
- No need to buffer change (Tris-Glycine-SDS buffer system)
- Optimized composition for a western transfer and blotting, brilliant blue and silver staining of gels

Storage

- Store at 4℃ for 1 year 

 

Kit Components

- Gradi-Gel I 2x Stacking Gel Buffer : 20 ml

- Gradi-Gel I 2x Running Gel Buffer : 100 ml 

 

References

 

• Kim et al. (2011) Effect of Saccharomyces cerevisiae ret1-1 Mutation on Glycosylation and Localization of the Secretome Molecules and Cells 2011; 31(2): 151-158.