EPD (Enhanced Peroxidase Detection) Western Reagent consists of enhanced chemiluminescent substrate for horseradish peroxidase (HRP) on immunoblots.
Storage ConditionStore at 2-8°C, and then stable for at least 6 months. Additional reagents required- Phosphate-buffered saline (PBS) : 80 mM Na₂HPO₄, 20 mM NaH₂PO₄, pH7.5, 100 mM NaCl- Tris-buffered saline (TBS) : 20 mM Tris-HCl, pH7.5, 137 mM NaCl- Tween-20- PBS-T or TBS-T : 0.05% to 1% of Tween-20 in PBS or TBS- Blocking reagents (BSA or non-fat dried milk) : usually 1 – 5% in PBS-T or TBS-T Primary antibody and HRP-secondary antibody ; It is recommended that the antibody dilution should be optimized empirically to maximize signal to noise Critical Parameters1. Proper dilution of primary and secondary antibodies for the best results.
2. Proper blocking and washing to minimize nonspecific signals.
1. EPD Substrate Solution A 100 ml and Solution B 100 ml.
2. Rapid protocol