PicoEPD (Enhanced Peroxidase Detection) Western Reagent consists of a highly sensitive and stable chemiluminescent substrate for horseradish peroxidase (HRP) on immunoblots.

The extremely intense signal output and stable emission of blue light for relatively long time (<12 hr) of PicoEPD substrate enables the detection of picogram quantities of antigen in immunoblot and direct or indirect South/Northern blot using HRP-conjugated polynucleotide probe.

Storage Condition
- Store at 2-8°C, and then stable for at least 6 months.

Additional reagents required
- Phosphate-buffered saline (PBS) : 80 mM Na₂HPO₄, 20 mM NaH₂PO₄, pH7.5,100 mM NaCl
- Tris-buffered saline (TBS) : 20 mM Tris-HCl, pH7.5, 137 mM NaCl
- Tween-20
- PBS-T or TBS-T : 0.05% to 1% of Tween-20 in PBS or TBS
- Blocking reagents (BSA or non-fat dried milk) : usually 1 – 5% in PBS-T or TBS-T Primary antibody and HRP-secondary antibody ; It is recommended that the antibody dilution should be optimized empirically to maximize signal to noise

Critical Parameters
1. Proper dilution of primary and secondary antibodies for the best results.

2. Proper blocking and washing to minimize nonspecific signals. 

 

 

Kit Components
1. PicoEPD Substrate Solution A 100 ml and Solution B 100 ml.
2. Rapid protocol