Description
The 100 bp DNA Ladder Marker is a mixture of specially designed double-stranded DNA fragments for determining the exact size of PCR products and engineered DNA fragments. The 100 bp DNA Ladder Marker consists of 12 DNA fragments ranging in size from 100 to 1,000 bp in 100 bp increment, and additional 1,500 and 2,000 bp fragment. For easy size reference on the gel electrophoresis, the 500 bp and 1,000 bp are two to three times more brighter than the other bands. The 100 bp DNA Ladder Marker is supplied in a ready-to-use format. This ladder marker can be stained with ethidium bromide or any other known DNA staining methods.
Storage Buffer
65 ㎍ in 0.5 ㎖ of 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 5% Glycerol, 0.005% Bromophenol Blue, 0.005% Xylene Cyanol and 0.001% SDS
Usage Information
Concentration : 130 ng/㎕ (65 ㎍/0.5 ㎖)
Recommended loading : 3 - 5 ㎕ (ready-to-use)
Range : 100 – 2,000 bp
Number of bands : 12
Recommended Storage Condition
-20 ℃ for 2 years
4 ℃ for 6 months
Room temperature (20-25 ℃) for 2 months