The 100bp DNA ladder marker is a mixture of specially designed double-stranded DNA fragments for determining the exact size of PCR products and engineered DNA fragments.
The 100bp DNA ladder marker consists of 11 DNA fragments ranging in size from 100 to 1,000 bp in 100 bp increment, and additional 1,500 bp fragment.
For easy size reference on the gel electrophoresis, the 500 bp and 1,000 bp are two to three times more brighter than the other bands.
This ladder marker can be stained with ethidium bromide or any other known DNA staining methods.
The 33 ㎍ of marker DNA is contained in 250 ㎕ TE buffer. Before start, optimal concentration and loading volume should be empirically determined by users to get the best result.
This product is supplied with 1 ㎕ of 6x gel loading buffer to dilute marker DNA.
Storage Buffer
- Marker DNA : 33 ㎍ in 250 ㎕ TE buffer
- 6x Gel loading buffer : 60 mM Tris-HCl, pH8.0, 6 mM EDTA, 30% Glycerol, 0.03% Bromophenol Blue, and 0.03% Xylene Cyanol
Recommended Storage Condition
- -20℃ for 2 years
- 4℃ for 6 months
- Room temperature (20-25℃) for 2 months
Usage Information
- Recommended loading : 100-500 ng/well according to the gel size, well size, and running length.
- Range : 100 – 1,500 bp
- Number of bands : 11
