The 1Kbp DNA ladder marker (supercoil) is a mixture of specially designed double-stranded and supercoiled DNAs for determining the exact size of cloned plasmid vectors in a supercoiled form.
The 1Kbp DNA ladder marker (supercoil) consists of 7 DNAs ranging in size from 2 to 10 Kbp.The 5 Kbp band is double in intensity and serves as a reference indicator.The 1Kbp DNA ladder marker (supercoil) is supplied in a ready-to-use format, premixed with loading buffer and tracking dyes (xylene cyanol and bromophenol blue).This ladder marker can be stained by ethidium bromide or any other known DNA staining methods.The ladder marker can not be used for quantitative purpose.Storage Buffer- Marker DNA : 20 ㎍ in 0.5 ㎕ of 10 mM Tris-HCl, pH8.0, 1 mM EDTA, 5% Glycerol, 0.005% Bromophenol Blue, and 0.005% Xylene CyanolRecommended Storage Condition- -70℃ for 2 year- -20℃ for 1 year- Just after opening the tube, aliquot the product into fresh tubes as soon as possible- Avoid repeated freezing and thawingUsage Information- Concentration : 200 ng/5 ㎕ (20 ㎍)- Recommended loading : 5-10 ㎕ (ready-to-use)- Range : 2,000 – 10,000 bp- Number of bands : 7
