The 200bp step ladder marker is a mixture of double-stranded DNA fragments from 200 to 3,000 bp in 200 bp step increment.
For easy size reference on the gel electrophoresis, the 600 bp, 2,000 bp, and 3,000 bp are made to be more brighter than the other bands.The 200bp step ladder marker is supplied in a ready-to-use format.This ladder marker can be stained with ethidium bromide or any other known DNA staining methods.Storage Buffer- Marker DNA : 100 ㎍ in 0.5 ㎕ of 10 mM Tris-HCl, pH8.0, 1 mM EDTA, 5% Glycerol, 0.005% Bromophenol Blue, and 0.005% Xylene CyanolRecommended Storage Condition- -20℃ for 2 year- 4℃ for 6 months- Room temperature (20-25℃) for 2 monthsUsage Information- Concentration : 1,000 ng/5 ㎕ (100 ㎍/0.5 ㎕)- Recommended loading : 2-5 ㎕ (100-200 lanes, ready-to-use)- Range : 200 bp step increase to 3 kbp
