The 100bp step ladder marker is a mixture of double-stranded DNA fragments from 100 to 3,000 bp in 100 bp step increment.
For easy size reference on the gel electrophoresis, the 500 bp, 1,000 bp, 2,000 bp, and 3,000 bp are made to be more brighter than the other bands.The 100bp step ladder marker is supplied in a ready-to-use format.This ladder marker can be stained with ethidium bromide or any other known DNA staining methods.Storage Buffer- Marker DNA : 100 ㎍ in 0.5 ㎕ of 10 mM Tris-HCl, pH8.0, 1 mM EDTA, 5% Glycerol, 0.005% Bromophenol Blue, and 0.005% Xylene CyanolRecommended Storage Condition- -20℃ for 2 year- 4℃ for 6 months- Room temperature (20-25℃) for 2 monthsUsage Information- Concentration : 1,000 ng/5 ㎕ (100 ㎍/0.5 ㎕)- Recommended loading : 2-5 ㎕(100-200 lanes, ready-to-use)- Range : 100 bp step increase from 100 bp to 3 kbp
