The 50bp step ladder marker is a mixture of double-stranded DNA fragments from 50 to 3,000 bp in 50 bp step increment.
For easy size reference on the gel electrophoresis, the 200 bp, 400 bp, 600 bp, 1,200 bp and 3,000 bp are made to be more brighter than the other bands.The 50bp step ladder marker is supplied in a ready-to-use format.This ladder marker can be stained with ethidium bromide or any other known DNA staining methods.Storage Buffer- Marker DNA : 150 ㎍ in 0.5 ㎕ of 10 mM Tris-HCl, pH8.0, 1 mM EDTA, 5% Glycerol, 0.005% Bromophenol Blue, and 0.005% Xylene CyanolRecommended Storage Condition- -20℃ for 2 year- 4℃ for 6 months- Room temperature (20-25℃) for 2 monthsUsage Information- Concentration : 1,500 ng/5 ㎕ (150 ㎍/0.5 ㎕)- Recommended loading : 2-5 ㎕ (100-200 lanes, ready-to-use)- Range : 50 bp step increase from 50 bp to 3 kbp
