Source

- Acinetobacter calcoaceticus.

Reaction Conditions
- Buffer 4. 20 mM Tris-acetate, pH7.9, 50 mM potassium acetate, 10 mM magnesium acetate, 1 mM DTT. Incubate at 37℃.

Concentration & Storage Condition
- Store at -20℃. 5 unit/㎕ in 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol.

Heat Inactivation Condition
- 80℃ for 20 min.

Enzyme Dilution
- Dilute with Storage Buffer.

QC Tests
- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.

Methylase Sensitivity

- Blocked by overlapping CpG methylation (Cleavage of mammalian genomic DNA is blocked). 

 

Note

- Acc I requires at least 13 base pairs beyond the end of its recognition sequence to cleave efficiently.