- Acinetobacter calcoaceticus.
Source Reaction Conditions- Buffer 4. 20 mM Tris-acetate, pH7.9, 50 mM potassium acetate, 10 mM magnesium acetate, 1 mM DTT. Incubate at 37℃. Concentration & Storage Condition- Store at -20℃. 5 unit/㎕ in 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol. Heat Inactivation Condition- 80℃ for 20 min. Enzyme Dilution- Dilute with Storage Buffer. QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests. Methylase Sensitivity
- Blocked by overlapping CpG methylation (Cleavage of mammalian genomic DNA is blocked).
- Acc I requires at least 13 base pairs beyond the end of its recognition sequence to cleave efficiently.