- Aquaspirillum serpens.
Source Reaction Conditions- Buffer 3. 50 mM Tris-HCl, pH 7.9, 100 mM NaCl, 10 mM MgCl₂, 1 mM DTT. Incubate at 37℃. Concentration & Storage Condition- Store at -20℃. 10 unit/㎕ in 10 mM Tris-HCl, pH 7.5, 500 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol. - 65℃ for 20 min.
Heat Inactivation Condition
- Dilute with Storage Buffer.
QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests. Methylase Sensitivity
- Not sensitive to dam, dcm or CpG methylation.
- Low salt, high glycerol (>5%) concentrations, pH > 8.0 or large excess of enzyme may result in star activity.