- Acinetobacter calcoaceticus.
Source Reaction Conditions- Buffer Acc III. 10 mM Tris-HCl, pH8.5, 100 mM NaCl, 10 mM MgCl₂, 1 mM DTT. Incubate at 65℃. Concentration & Storage Condition - Store at -20℃. 5 unit/㎕ in 10 mM Tris-HCl, pH 7.4, 100 mM KCl, 1 mM DTT, 1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol. Heat Inactivation Condition- 80℃ for 20 min. Enzyme Dilution- Dilute with Storage Buffer. QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests. Methylase Sensitivity
- Blocked by CpG methylation (Cleavage of mammalian genomic DNA is blocked).
- Incubate at 37℃ results in < 25% activity.