Source
- Acinetobacter calcoaceticus.Reaction Conditions- Buffer Acc III. 10 mM Tris-HCl, pH8.5, 100 mM NaCl, 10 mM MgCl₂, 1 mM DTT. Incubate at 65℃.Concentration & Storage Condition
Heat Inactivation Condition- 80℃ for 20 min.Enzyme Dilution- Dilute with Storage Buffer.QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.Methylase Sensitivity- Blocked by CpG methylation (Cleavage of mammalian genomic DNA is blocked).
Note
- Incubate at 37℃ results in < 25% activity.
