Source
- Arthrobacter protophormiae.Reaction Conditions- Buffer 3 + BSA. 50 mM Tris-HCl, pH 7.9, 100 mM NaCl, 10 mM MgCl₂, 1 mM DTT. Supplement with 100 ㎍/ml BSA. Incubate at 50℃.
Concentration & Storage ConditionHeat Inactivation Condition- 80℃ for 20 min.Enzyme Dilution- Dilute with Storage Buffer.
QC TestsMethylase Sensitivity- Not sensitive to dam, dcm or CpG methylation.
Note
- Incubation at 37℃ results in 50% activity.
