Source
- Diplococcus pneumoniae G41.Reaction Conditions- Buffer 4. 20 mM Tris-acetate, pH 7.9, 50 mM potassium acetate, 10 mM magnesium acetate, 1 mM DTT. Incubate at 37℃.Concentration & Storage Condition- Store at -20℃. 10 unit/㎕ in 10 mM Tris-HCl, pH 7.4, 400 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol.Heat Inactivation Condition- 80℃ for 20 min.Enzyme Dilution- Dilute with Storage Buffer.QC Tests
- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.
Note
- Dpn I is active only when its site is methylated.
