- Diplococcus pneumoniae G41.
Source Reaction Conditions- Buffer Dpn II. 50 mM Bis-Tris-HCl, pH 6.0, 100 mM NaCl, 10 mM MgCl₂, 1 mM DTT. Incubate at 37℃. Concentration & Storage Condition - Store at -20℃. 10 unit/㎕ in 10 mM Tris-HCl, pH 7.4, 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol. Heat Inactivation Condition- 65℃ for 20 min. Enzyme Dilution - Dilute with Storage Buffer. QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests. Methylase Sensitivity
- Blocked by overlapping CpG methylation (cleavage of mammalian genomic DNA is blocked). Blocked by dam methylation.
- pH > 6.5 may result in star activity