Source
- Diplococcus pneumoniae G41.Reaction Conditions- Buffer Dpn II. 50 mM Bis-Tris-HCl, pH 6.0, 100 mM NaCl, 10 mM MgCl₂, 1 mM DTT. Incubate at 37℃.Concentration & Storage Condition
Heat Inactivation Condition- 65℃ for 20 min.Enzyme Dilution
QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.Methylase Sensitivity- Blocked by overlapping CpG methylation (cleavage of mammalian genomic DNA is blocked). Blocked by dam methylation.
Note
- pH > 6.5 may result in star activity
