- Deinococcus radiophilus.
Source Reaction Conditions - Buffer 3 + BSA. 50 mM Tris-HCl, pH 7.9, 100 mM NaCl, 10 mM MgCl₂, 1 mM DTT. Supplement with 100 ㎍/ml BSA. Incubate at 37℃. Concentration & Storage- Store at -20℃. 10 unit/㎕ in 20 mM Tris-HCl, pH 7.4, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol. Heat Inactivation- Condition 65℃ for 20 min. Enzyme Dilution Dilute with Storage Buffer. QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests. Methylase Sensitivity
- Impaired by overlapping CpG methylation.
- Low salt, high glycerol (> 5%) concentrations, pH > 8.0 or large excess of enzyme may result in star activity.