Source
- Haemophilus influenzae Rd.Reaction Conditions- Buffer 2. 10 mM Tris-HCl, pH 7.9, 50 mM NaCl, 10 mM MgCl₂, 1 mM DTT. Incubate at 37℃.Concentration & Storage Condition- Store at -20℃. 20 unit/㎕ in 10 mM Tris-HCl, pH 7.4, 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 ㎍/ml BSA, 50% (v/v) glycerol.Heat Inactivation Condition- 65℃ for 20 min.Enzyme Dilution- Dilute with Storage Buffer.QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.Methylase Sensitivity- Not sensitive to dam, dcm or CpG methylation.
Note
- Low salt, high glycerol (>5%) concentrations, pH > 8.0 or large excess of enzyme may result in star activity.
