- Methanobacterium wolfeii.
Source Reaction Conditions- Buffer 3. 50mM Tris-HCl, pH 7.9, 100mM NaCl, 10mM MgCl2, 1mM DTT. Incubate at 60℃. Concentration & Storage Condition- Store at -20℃. 5 unit/㎕ in 10 mM Tris-HCl, pH 7.4, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol. Heat Inactivation Condition- No. Enzyme Dilution- Dilute with Storage Buffer.
QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.
- Blocked by some combinations of overlapping CpG methylation.
- Incubate at 37℃ results in 10% activity.