Source
- Streptomyces fimbriatus.Reaction Conditions- Buffer 4 + BSA. 20 mM Tris-acetate, pH 7.9, 50 mM potassium acetate, 10 mM magnesium acetate, 1 mM DTT. Supplement with 100 ㎍/ml BSA. Incubate at 50℃.Concentration & Storage Condition
Heat Inactivation Condition- No.Enzyme Dilution- Dilute with Storage Buffer.QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.Methylase Sensitivity- Blocked by some combinations of overlapping CpG methylation.
Note
- Incubation at 37 results in 10% activity.
