- Streptomyces fimbriatus.
Source Reaction Conditions- Buffer 4 + BSA. 20 mM Tris-acetate, pH 7.9, 50 mM potassium acetate, 10 mM magnesium acetate, 1 mM DTT. Supplement with 100 ㎍/ml BSA. Incubate at 50℃. Concentration & Storage Condition - Store at -20℃. 10 unit/㎕ in 20 mM Tris-HCl, pH 7.4, 300 mM NaCl, 5 mM 2-mercaptoethanol, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol, 0.15% Triton X-100. Heat Inactivation Condition- No. Enzyme Dilution- Dilute with Storage Buffer. QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests. Methylase Sensitivity
- Blocked by some combinations of overlapping CpG methylation.
- Incubation at 37 results in 10% activity.