- Sphaerotilus species.
Source Reaction Conditions- Buffer Ssp I. 100 mM Tris-acetate, pH 7.5, 50 mM NaCl, 10 mM MgCl₂, 0.025% Triton X-100. Incubate at 37℃. Concentration & Storage Condition- Store at -20℃. 10 unit/㎕ in 10 mM Tris-HCl, pH 7.4, 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol, 0.15% Triton X-100. Heat Inactivation Condition- 65℃ for 20 min. Enzyme Dilution - Dilute with Storage Buffer. QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests. Methylase Sensitivity
- Not sensitive to dam, dcm or CpG methylation.
- Low salt, high glycerol (>5%) concentrations, pH > 8.0 or large excess of enzyme may result in star activity.