Source
- Sphaerotilus species.Reaction Conditions- Buffer Ssp I. 100 mM Tris-acetate, pH 7.5, 50 mM NaCl, 10 mM MgCl₂, 0.025% Triton X-100. Incubate at 37℃.Concentration & Storage Condition- Store at -20℃. 10 unit/㎕ in 10 mM Tris-HCl, pH 7.4, 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol, 0.15% Triton X-100.Heat Inactivation Condition- 65℃ for 20 min.Enzyme Dilution
QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.Methylase Sensitivity- Not sensitive to dam, dcm or CpG methylation.
Note
- Low salt, high glycerol (>5%) concentrations, pH > 8.0 or large excess of enzyme may result in star activity.
