Source
- Thermus thermophilus.Reaction Conditions- Buffer 4 + BSA. 20 mM Tris-acetate, pH 7.9, 50 mM potassium acetate, 10 mM magnesium acetate, 1 mM DTT. Supplement with 100 ㎍/ml BSA. Incubate at 65℃.Concentration & Storage Condition- Store at -20℃. 10 unit/㎕ in 10 mM Tris-HCl, pH 7.5, 300 mM KCl, 1 mM DTT, 0.1 mM EDTA, 500 ㎍/ml BSA, 50% (v/v) glycerol.Heat Inactivation Condition- No.Enzyme Dilution- Dilute with Storage Buffer.QC Tests- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.Methylase Sensitivity- Not sensitive to dam, dcm or CpG methylation.
Note
- Incubation at 37℃ results in 10% activity.
