The BamH I Methylase recognizes 5´--GGATCC--3´ sequences and methylates internal cytosine (C⁴) residues.
Source- Recombinant gene from Bacillus amyloliquefaciens H.Applications- Blocking restriction endonuclease cleavage restricted by BamH I methylation.Reaction Conditions- 1x Methylase reaction buffer in the presence of 160 μM S-adenosylmethionine (SAM). Incubate at 37℃.Concentration & Storage Condition
Unit Definition- One unit is defined as the amount of enzyme required to fully protect against BamH I cleavage of 1 ㎍ of λ DNA in 1 hr at 37℃.
Heat Inactivation ConditionQC Tests
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.
