The Hae III methylase recognizes 5´--GGCC--3´ sequences and methylates internal cytosine (C⁵) residues.
Source- Recombinant gene from Haemophilus aegyptius. Applications- Blocking restriction endonuclease cleavage restricted by Hae III methylation. Reaction Conditions- 1x Methylase reaction buffer in the presence of 160 μM S-adenosylmethionine (SAM). Incubate at 37℃. - Store at -20℃. 10 unit/㎕ in 50 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 10 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol.
Concentration & Storage Condition
Unit Definition- One unit is defined as the amount of enzyme required to fully protect against Hae III cleavage of 1 ㎍ of λ DNA in 1 hr at 37℃. - 65℃ for 20 min.
Heat Inactivation Condition
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.