Poly(A) Polymerase catalyzes the addition of AMP to the 3´-hydroxyl terminus of RNA. Poly(A) Polymerase can add poly(C) and poly(U) to RNA, but does so with much-reduced efficiency. Poly(A) Polymerase is purified from a recombinant E.coli strain.
Concentration & Storage Condition - 1 unit/㎕. Store at -20℃. Storage Buffer- 25 mM Tris-HCl, pH 7.9, 500 mM NaCl, 1 mM DTT, 1 mM EDTA and 50% (v/v) glycerol. 10x Reaction Buffer- 500 mM Tris-HCl, pH 7.9, 2.5 M NaCl, 100 mM MgCl₂. Unit Definition- One unit incorporates 1 nmole of AMP into tRNA in 10 min at 37℃. QC Tests
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.