Phi29 DNA Polymerase is one of the enzymes showing strong strand displacement DNA synthetic activity. The polymerase has an inherent 3' to 5´ proof-reading exonuclease activity. The polymerase gene from the Bacillus subtilis phage phi29 (Φ29) was overexpressed and purified from E.coli strain.
Features- Extreme strand displacement : best fit for RCA (rolling circle amplification) and WGA by MDA (whole genome amplification by multiple displacement amplification- High fidelity : 3' to 5´ proof-reading exonuclease activity- Extreme processivity : up to 70 kbp Concentration & Storage Condition- 10 unit/㎕. Store at -20℃. Storage Buffer- 10 mM Tris-HCl, pH 7.5, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol. 10x Reaction Buffer- 500 mM Tris-HCl, pH 7.5, 100 mM (NH₄)₂SO₄, 100 mM MgCl₂, 40 mM DTT. Unit Definition- One unit is defined as the amount of enzyme required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C QC Tests
- Activity, exo and endonuclease activity test, DNA contamination test, SDS-PAGE purity, performance tests.