T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded DNA or RNA onto the 5´-phosphoryl termini of single-stranded DNA or RNA. T4 RNA Ligase also catalyzes the addition of [5´-32P] nucleoside 3´,5´-bis (phosphate) onto single-stranded RNA. T4 RNA Ligase is purified from a recombinant E.coli strain.
Concentration & Storage Condition- 10 unit/㎕. Store at -20℃. Storage Buffer- 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween 20, 50% glycerol. 10x Reaction Buffer- 500 mM Tris-HCl, pH 7.8, 100 mM MgCl₂, 50 mM DTT, 10 mM ATP. Unit Definition- One unit catalyzes the formation of 1 nmole of [5´-³² P]rA₁₄-₂₀ into a phosphatase-resistant form in 30 min at 37°C in a total volume of 30 ㎕. The reaction conditions are: 50 mM Tris-HCl, pH 7.8, 10 mM MgCl₂, 5 mM DTT, 1 mM ATP and 10 mM [5´-³²P]rA₁₄-₂₀. QC Tests
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.