Lambda Exonuclease catalyzes the removal of 5´-mononucleotides from duplex DNA (5´ to 3´). The preferred substrate is 5´-phosphorylated double-stranded DNA, although it will also degrade single-stranded and non-phosphorylated substrates at a greatly reduced rate. Lambda Exonuclease is unable to initiate DNA digestion at nicks or gaps.
Concentration & Storage Condition- 5 unit/㎕. Store at -20℃. Storage Buffer- 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50 μg/ml BSA and 50% glycerol. 10x Reaction Buffer- 670 mM Glycine-KOH, pH 9.4, 25 mM MgCl₂ and 500 ㎍/ml BSA. - One unit is defined as the amount of enzyme required to produce 10 nmole of acid-soluble dNTP from double-stranded substrate in a total reaction volume of 50 ㎕ in 30 min at 37℃ in 1x Lambda Exonuclease reaction buffer with 1 ㎍ sonicated duplex [3H]-DNA.
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.