E.coli RNase III catalyzes the long double-stranded RNA into a heterogeneous short (18-25 bp) interfering RNAs (siRNA) suitable for RNA interference in mammalian cells. 1 unit of RNase III is sufficient to convert 1 ㎍ of dsRNA into siRNA suitable for RNA interference in mammalian cells. RNase III is purified from a recombinant E.coli strain.
Concentration & Storage Condition- 1 unit/㎕. Store at -20℃. - 10 mM Tris-HCl, pH8.0, 500 mM NaCl, 1 mM DTT, 0.5 mM EDTA, 50% glycerol.
- 500 mM Tris-HCl, pH 7.5, 10 mM DTT, 100 mM MnCl₂.
10x Reaction Buffer
Unit Definition- One unit is the amount of enzyme required to digest 1 ㎍ of dsRNA to siRNA in 20 min at 37°C in a total reaction volume of 50 ㎕. QC Tests
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.