Exonuclease III catalyzes the stepwise removal of mononucleotides starting from a 3´-OH at nicks, blunt ends, recessed ends and 3´-overhangs of less than 4 bases. Exonuclease III is used in conjunction with S1 nuclease for unidirectional deletion of sequences from

the termini of DNA fragments to construct a series of deletion mutants.
Exonuclease III is purified from a recombinant E.coli strain.

Concentration & Storage Condition
- 100 unit/㎕. Store at -20℃.

Storage Buffer
- 20 mM Tris-HCl, pH 7.5, 1 mM DTT, 100 mM KCl, 50% glycerol.

10x Reaction Buffer
- 660 mM Tris-HCl, pH 8.0, 6.6 mM MgCl₂.

Unit Definition
- One unit is defined as the amount of enzyme required to produce 1 nmole of acid-soluble nucleotides from double-stranded DNA within 30 min at 37°C.

QC Tests

- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.