Exonuclease III catalyzes the stepwise removal of mononucleotides starting from a 3´-OH at nicks, blunt ends, recessed ends and 3´-overhangs of less than 4 bases. Exonuclease III is used in conjunction with S1 nuclease for unidirectional deletion of sequences fromthe termini of DNA fragments to construct a series of deletion mutants.Exonuclease III is purified from a recombinant E.coli strain.
Concentration & Storage Condition- 100 unit/㎕. Store at -20℃. Storage Buffer- 20 mM Tris-HCl, pH 7.5, 1 mM DTT, 100 mM KCl, 50% glycerol. 10x Reaction Buffer- 660 mM Tris-HCl, pH 8.0, 6.6 mM MgCl₂. Unit Definition- One unit is defined as the amount of enzyme required to produce 1 nmole of acid-soluble nucleotides from double-stranded DNA within 30 min at 37°C. QC Tests
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.