Micrococcal Dual Nuclease is a non-specific endo/exonuclease that effectively digests both DNA and RNA substrates. The enzyme is active for double-stranded, single-stranded, circular and linear nucleic acids in the pH range of 7-10.Micrococcal nuclease gene is obtained from Staphylococcus aureus and recombinant enzyme is expressed and purified from E.coli strain.
Concentration & Storage Condition- 1 Kunit/㎕, Store at -20℃. 10x Reaction Buffer- 500 mM Tris-HCl, pH7.9, 50 mM CaCl₂. - 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM EDTA and 50% glycerol.
- One unit is defined as the amount of enzyme required to digest 1 µg of genomic DNA in 15 minutes at 37°C.
- Activity, SDS-PAGE purity, performance tests.