Bacterial Alkaline Phosphatase catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vector DNA by removing phosphate groups from both 5´-termini and may also be used for the dephosphorylation of 5´ phosphorylated ends of DNA or RNA for subsequent labeling with [γ-³²P]ATP and T4 Polynucleotide Kinase. Bacterial Alkaline Phosphatase is purified from a recombinant E.coli strain.
Concentration & Storage Condition- 0.5 unit/㎕. Store at -20℃. Storage Buffer- 50 mM Tris-HCl, pH 8.0, 100 mM KCl, 1 mM MgSO₄, 50% glycerol. 10x Reaction Buffer- 500 mM Tris-HCl, pH 9.0, 10 mM MgCl₂. Unit Definition- One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1 μmol of 4-nitrophenyl phosphate per min at 37°C in 1 M diethanolamine, 10.9 mM 4-nitrophenyl phosphate, 0.5 mM MgCl₂, pH 9.8. QC Tests
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.