5'-3' Exonuclease (Exo53) catalyzes the removal of 5´ mononucleotides from duplex DNA (5' to 3´ exonuclease avtivity). Unlike lambda exonuclease, Exo53 degrades both 5´-phosphorylated and 5'-dephosphorylated double stranded DNA. Exo53 is unable to initiate DNA digestion at nicks or gaps. Exo53 is purified from E.coli strain containing gene of exonuclease domain of E.coli DNA pol I.

Concentration & Storage Condition
- 10 unit/㎕, Store at -20℃.

10x Reaction Buffer
- 100 mM Tris-HCl, pH7.9, 500 mM NaCl, 100 mM MgCl₂, 10 mM DTT.

Storage Buffer
- 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% glycerol.

Unit Definition
- One unit is defined as the amount of enzyme required to produce 1 nmole of acid-insoluble nucleotides from double-stranded DNA within 1 hr at 37°C.

QC Tests

- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.