rDNase I (recombinant) endonucleolytically cleaves double-stranded DNA to 5´-phosphodinucleotide and 5´-phosphooligonucleotide end-products. rDNase I (recombinant) is overexpressed and purified from E.coli strain.
Concentration & Storage Condition- 100 unit/㎕. Store at -20℃. Storage Buffer - 10 mM Tris-HCl, pH7.5, 10 mM MgCl₂, 10 mM CaCl₂, 50% glycerol. 10x Reaction Buffer- 100 mM Tris-HCl, 7.5, 25 mM MgCl₂, 5 mM CaCl₂. Unit Definition - 1 unit is defined as the amount of enzyme required to completely degrade 1 ㎍ of DNA for 10 min at 37°C in 50 ㎕ buffer containing 40 mM Tris-HCl, pH 7.9, 10 mM NaCl, 6 mM MgCl₂, 10 mM CaCl₂. QC Tests
- Activity, SDS-PAGE purity, performance tests.