Endonuclease IV is a metalloenzyme that functions in vivo to repair free-radical damage in DNA and cleaves the phosphodiester bond 5' to the lesion generating a hydroxyl group at the 3'-terminus and a deoxyribose 5´-phosphate at the 5´ terminus. The enzyme also has a 3´ -diesterease activity and possesses a 3‘ to 5' exonuclease activity. Its progression on substrates is sensitive to ionic strength, metal ions, EDTA, and reducing conditions. Endonuclease IV is expressed and purified from E.coli.

Applications
- Studies of DNA damage and repair
- DNA structure and SNP analysis
- Antitumor drug evaluation

Reagents Supplied & Storage Condition
- Endonuclease IV : 10 unit/㎕, Store at -20°C.
- 10x Endonuclease IV Reaction Buffer : Store at 4°C.

Reaction Condition
- Endonuclease IV in 1X Endonuclease IV Reaction Buffer. Incubate at 37°C.

10x Reaction Buffer
- 500 mM Tris-HCl (pH 7.9), 1 M NaCl, 100 mM MgCl₂, 10 mM DTT

Storage Buffer
- 10 mM Tris-HCl (pH 7.4), 250 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 ㎍/ml BSA, 50% Glycerol, 0.15% Triton X-100

Unit Definition
- One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34-mer oligonucleotide duplex containing a single AP site* in a total reaction volume of 10 µl in 1 hour at 37°C.

QC Tests

- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.