Step-view™ 20kD pre-stained marker is a dual color pre-stained size marker. It is a mixture of 8 recombinant purified and pre-stained polypeptides whose molecular weights are well-adjusted. Step-view™ 20kD pre-stained marker is available in a ready-to-use formula (no need to boil before use), and is suitable for sizing proteins between 20,000 and 140,000 Da by SDS-PAGE.
A blue or orange chromophore is covalently bound to proteins, and 8 pre-stained proteins are visible during electrophoresis or electrophoretic transfer from the gel to the membrane. The 40 and 100 kDa proteins labeled with a orange chromophore enables easy discrimination. The markers are not intended for use in quantitative analysis.Because the coupling of chromophore to the proteins affects their apparent molecular weights in SDS-PAGE relative to un-stained proteins, un-stained protein standard (Cat. No. EBM-1020) should be used for accuracy >95%.
- 5 ㎕/lane for a mini-gel (10 x 8㎠, 0.75㎜ or 1㎜ thick)- 10-15 ㎕/lane for a large-gel (30 x 20㎠, 1㎜ or 1.5㎜ thick)
Recommended loading volume
Recommended storage condition- Store at -20℃ for one year
※ Please aliquot in a small volume for prevention of repeated freezing & thawing