Broad-range Protein Markers are useful for SDS-PAGE analysis of your proteins.The each marker is a mixture of 10 recombinant purified proteins whose molecular weights are well-defined.The markers are supplied in a ready-to-use formula (no need to boil before use), and is suitable for sizing proteins between 7,000 and 240,000 Da by SDS-PAGE.Chromophores are covalently linked to proteins in pre-stained markers, and 10 pre-stained proteins are visible during electrophoresis or electrophoretic transfer from the gel to the membrane.The 25 and 70 kDa proteins play a ruler function for easy identification of banding.Because coupling of chromophores to the proteins in pre-stained markers affects their apparent molecular weights in SDS-PAGE relative to un-stained proteins, un-stained protein standard (Cat. No. EBM-1034) should be used for accuracy >95%.
Recommended loading volume - 5 ㎕/lane for a mini-gel (10x8 ㎠, 0.75 ㎜ or 1 ㎜ thick) - 10-15 ㎕/lane for a large-gel (30x20 ㎠, 1 ㎜ or 1.5 ㎜ thick) - 2-5 ㎕/lane for a western transfer onto PVDF or NC membranes Recommended storage condition - Store at -20℃ for one year
※ Please aliquot in a small volume for prevention of repeated freezing & thawing