Broad-range Protein Markers are useful for SDS-PAGE analysis of your proteins.
The each marker is a mixture of 10 recombinant purified proteins whose molecular weights are well-defined.The markers are supplied in a ready-to-use formula (no need to boil before use), and is suitable for sizing proteins between 7,000 and 240,000 Da by SDS-PAGE.The 25 and 70 kDa proteins play a ruler function for easy identification of banding by two times more concentrations.
Recommended loading volume- 5 ㎕/lane for a mini-gel (10x8 ㎠ 0.75 ㎜ or 1 ㎜ thick)- 10-15 ㎕/lane for a large-gel (30x20 ㎠, 1 ㎜ or 1.5 ㎜ thick)- 2-5 ㎕/lane for a western transfer onto PVDF or NC membranes - Store at -20℃ for one year
Recommended storage condition
※ Please aliquot in a small volume for prevention of repeated freezing & thawing