West-view western markers are brand-new, innovative true protein size markers for a western blot analysis.You can quickly and easily visualize discrete bands on your western blot only by a simple application of marker with your samples. You can get superior results, without further processing like adding extra reagents as described by many other suppliers.All the highly purified recombinant proteins are designed to bind immunoglobulins with high affinity in a denatured form, thus marker proteins directly bind to primary or secondary antibodies in your western blotting.You can use it in a chemiluminescent or chromogenic detection methods of your choice. It's compatible with most immunodetection methods, including horseradish peroxidase or alkaline phosphatase systems. You can get sharp, clear bands and precise, reproducible molecular weight estimation directly on the western blot as shown on the figures.West-view western markers can also be used for optimizing your western transfer condition, verification of antibody conjugated peroxidase intactness, and controlling the detection system.In case, size dependent uneven band’s densities of western size marker in western blotting indicate the inappropriate transfer of your proteins to the membrane, and further requires adjusting protein transfer condition.No marker bands in western blot may indicate functional problems in your detection system.
Main Features- Easy-to-use procedure- Direct marker visualization on western blots (x-ray film, membranes, or on chemiluminescence imager)- Accurate protein molecular weight estimation directly on western blots- Useful for optimizing western transfer, antibody intactness, and detection system Storage Buffer- 62.5 mM Tris-HCl, pH 6.8, 1% β-mercaptoethanol, 35% glycerol, 1% SDS, and 0.01% bromophenol blue. Recommended loading volume- 5 ㎕ of the marker solution is recommended on a 10 x 8㎠ mini-gel. Sufficient reagents are provided to perform 50 applications. Storage
- Upon arrival, store at -20°C. To avoid repeated freezing and thawing, aliquot in small volumes and store.
- 250 ㎕ of West-view western markers in a ready-to-use format (no need to boil before use).