1) Gel extraction
The DokDo-Prep gel extraction kit is designed for the purification of DNA from standard agarose gel (TAE/TBE).
The DokDo-Prep gel extraction kit (spin-type) uses spin column containing silica membrane to purify the DNA fragment.This enables extraction of DNA fragments from both TAE and TBE buffer-containing agarose gels without additional solutions or modifications to the protocol.Fragments can be also extracted from low-melting-point agarose.- DNA fragments in the size range of 100 bp to 10 kbp can be extracted from gels.- The procedure is brief, allowing completion of the protocol within 15 to 20 min.- The recovery of DNA is 70~80%.- The maximum DNA binding capacity is 30~40 ㎍/column.
- Purified DNA is suitable for a variety of applications, including fluorescent DNA sequencing, transformation, restriction mapping, cloning, and labeling.
2) PCR Purification
The DokDo-Prep PCR purification kit is a convenient system for a fast and reliable purification of double- or single-stranded PCR products.
The method uses DokDo-Prep PCR purification technology to recover DNA bands 100 bp - 10 kbp in size free from oligonucleotides, nucleotides, and polymerase in recovery
efficiency exceeding 90 to 95%.
Binding conditions are adjusted by addition of a specially formulated Binding Buffer, and the sample is applied to DokDo-Prep spin column.
Purified DNA fragment is ready for restriction enzyme digestion, labeling, ligation, transformation, and sequencing reactions.
- Spin Column 200 ea with collection tubes (2 ml)- Gel/PCR Purification Buffer 100 ml x 2- Wash Buffer 20 ml x 2- Elution Buffer 15 ml
- Manual 2 ea