EBD-1009 Gel/PCR Purification kit

1. Gel extraction

 

The Gel Extraction provides two simple techniques for separating DNA from agarose.

 

Both techniques involve as a first step, the solubilization of agarose in the chaotropic salt, and the second step is either binding of the DNA to glass absorbent (glass binding

 

method). DNA generally binds to silica in the presence of high concentration of chaotropic salt. Contaminants such as salts and soluble macromolecular components are

 

removed by a simple washing step. DNA/silica binding is abolished when salt concentration is lowered.

 

Since the DNA is eluted with either water or a low salt elution buffer, it can be used immediately in subsequent reactions without precipitation or other further manipulation.

 

2. PCR Purification

 

The Dokdo-PrepTM Gel/PCR Purification Kit is a convenient system for fast and reliable purification of double- or single-stranded PCR products.

 

The method uses Dokdo-PrepTM PCR purification technology to recover DNA bands 100 bp - 10 kb free from oligonucleotides, nucleotides,

 

and polymerase in yields exceeding 90 to 95%.

 

Binding conditions are adjusted by addition of a specially formulated buffers, and the sample is simply applied to spin column after mixing PCR products with Binding Buffer.

 

Purified DNA fragment is ready for restriction enzyme digestion, labeling, ligation, transformation, and sequencing reactions. 

 

 

 

 

Kit Contents

 

- Spin Column 200 ea with collection tubes (2 ml)

- Gel/PCR Purification Buffer 100 ml x 2
- Wash Buffer 20 ml x 2
- Elution Buffer 15 ml

- Manual 2 ea (EBD-1004, EBD-1005)