1) Gel extraction

The DokDo-Prep gel extraction kit is designed for the purification of DNA from standard agarose gel (TAE/TBE).

The DokDo-Prep gel extraction kit (spin-type) uses spin column containing silica membrane to purify the DNA fragment.

This enables extraction of DNA fragments from both TAE and TBE buffer-containing agarose gels without additional solutions or modifications to the protocol.
Fragments can be also extracted from low-melting-point agarose.

- DNA fragments in the size range of 100 bp to 10 kbp can be extracted from gels.
- The procedure is brief, allowing completion of the protocol within 15 to 20 min.
- The recovery of DNA is 70~80%.
- The maximum DNA binding capacity is 30~40 ㎍/column.

- Purified DNA is suitable for a variety of applications, including fluorescent DNA sequencing, transformation, restriction mapping, cloning, and labeling. 

 

 

2) PCR Purification

The DokDo-Prep PCR purification kit is a convenient system for a fast and reliable purification of double- or single-stranded PCR products.

The method uses DokDo-Prep PCR purification technology to recover DNA bands 100 bp - 10 kbp in size free from oligonucleotides, nucleotides, and polymerase in recovery

efficiency exceeding 90 to 95%.

Binding conditions are adjusted by addition of a specially formulated Binding Buffer, and the sample is applied to DokDo-Prep spin column.

Purified DNA fragment is ready for restriction enzyme digestion, labeling, ligation, transformation, and sequencing reactions. 

Kit Contents

- Spin Column 200 ea with collection tubes (2 ml)
- Gel/PCR Purification Buffer 100 ml x 2
- Wash Buffer 20 ml x 2
- Elution Buffer 15 ml

- Manual 2 ea