10X TBE (Tris-Borate-EDTA Buffer) is used for polyacrylamide and agarose gel electrophoresis.
TBE was originally used at a working strength of 1X for polyacrylamide gel and 0.5X for agarose gel electrophoresis.Premixed electrophoresis buffers eliminate variables which can distort data and give unreliable results.10X Composition- 890 mM Tris base- 890 mM Boric acid- 20 mM EDTA- pH at 25°C (1X): 8.2-8.4Recommended Storage Condition- Store at room temperature (22–25°C)Usage Information (applications)
- Electrophoresis buffer for polyacrylamide and agarose gel electrophoresis.